Antibiotic (carbadox) effects on the swine gut microbiota - SRA

SRX2664128: Antibiotic (carbadox) effects on the swine gut microbiota
1 LS454 (454 GS FLX Titanium) run: 142,087 spots, 106.4M bases, 70.1Mb downloads

Design: DNA and RNA samples were extracted from the same fecal samples from piglets n = 12 from two sows that were divided into two treatment groups non-amended feed or 50 g carbadox / ton feed as a previous study {Looft:2014bj}. Prior to nucleic acid extraction, an artificial RNA spike-in name the vector used, how linearized, purification, size was added to the fecal material as described previously {Gifford:2011eo} to normalize transcript counts by g fecal material. RNA and DNA were extracted from the same fecal material using the PowerMag Microbiome RNA/DNA isolation kit following the manufacturers instructions. Bacterial community DNA was sequenced on a Roche GS-FLX instrument with Titanium chemistry 454 Life Sciences

Submitted by: Iowa State University

Study: Swine feces Targeted Locus (Loci)

PRJNA237795 • SRP038757 • All experiments • All runs

show Abstracthide Abstract

Taxonomic survey (16S rRNA gene) of bacterial membership in the pig intestinal tract during in-feed antibiotics (carbadox) and after withdrawal.

Sample: MIMS Environmental/Metagenome sample from gut metagenome

SAMN02645055 • SRS560630 • All experiments • All runs

Organism: gut metagenome

Library:

Name: 190_2_feces_mg

Instrument: 454 GS FLX Titanium

Strategy: WGS